| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076292 |
| Chromosome: | chromosome 6 |
| Location: | 4817843 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280000 | non-conserved protein of unknown function | 3'UTR | |
| Cre06.g280050 | ROC86,XRN1 | (1 of 1) K12618 - 5'-3' exoribonuclease 1 (XRN1, SEP1, KEM1); 5' to 3' exoribonuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTCCCCCCAGTTGGCTACCGACCTTCCACAACCGTGCAACAGGTCTT |
| Internal bar code: | TATGTCGCGACAAAGAATTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6239 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCATTCATTGGCGTACGG |
| Suggested primer 2: | TAGAATGCAGGCACAGGTGG |