| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076327 |
| Chromosome: | chromosome 1 |
| Location: | 387144 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002300 | CYN4,CYN19B,CYN19-2,ROC1 | (1 of 4) K01802 - peptidylprolyl isomerase (E5.2.1.8); Cyclophilin 19-2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAACAAAATAATTGTGTTACAGCGGAGCTTAAAATGCAGTTCTCAAGG |
| Internal bar code: | AGTGTGCGTGAAACGCTAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1432 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGGCATGGTATCAAAAG |
| Suggested primer 2: | ACTCACGTTGGGGATCACAC |