| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.076404 |
| Chromosome: | chromosome 6 |
| Location: | 6508708 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294200 | (1 of 1) 6.5.1.2 - DNA ligase (NAD(+)) / Polynucleotide ligase (NAD+) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGCAGCAGACGTGGGCGCCGACGTCGCCGCGCCAGGCGGCATGGTGA |
| Internal bar code: | ACAACGCTTACCGAGGGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGGTAAAGCACACGGTG |
| Suggested primer 2: | TTCACTCTGAACGTCGGCAA |