Insertion junction: CLIP2.076545_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076545
Chromosome:chromosome 2
Location:138575
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g074150 HKR1,COP5 (1 of 1) IPR001054//IPR001425//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006//IPR013761//IPR029730//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // Archaeal/bacterial/fungal rhodopsin // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily // Sterile alpha motif/pointed domain // Archaeal/bacterial/fungal rhodopsin-like // Nucleotide cyclase; Histidine kinase rhodopsin 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGTCGTGCTGCCGCACCGCTCGTCCGCACGCCAGCACAACAAACAGA
Internal bar code:AGTTTAACCTGTATGAGTGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:901
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTGTTTGGTGTGTACTG
Suggested primer 2:CGAACTCGTACCGTACCTCG