| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076652 |
| Chromosome: | chromosome 10 |
| Location: | 6127788 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462200 | HDA17,SRTA1,SRTA | Sir2-like NADH dependent histone deacetylase; (1 of 1) K11416 - mono-ADP-ribosyltransferase sirtuin 6 (SIRT6, SIR2L6) | 3'UTR |
| Cre10.g462250 | REF1 | RNA Export Factor; (1 of 1) K12881 - THO complex subunit 4 (THOC4, ALY) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGAGGACGGCAGCAAGCAAGTGCTGCACCCTCGCAGGCGGAGTGTTG |
| Internal bar code: | AGAGAATCTGGATTATGCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2287 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGAGGTGTCAGTGACCTG |
| Suggested primer 2: | GGGCTGCATGGACTGTACTT |