| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076725 |
| Chromosome: | chromosome 11 |
| Location: | 2160710 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467738 | (1 of 8) K13348 - protein Mpv17 (MPV17) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCGAACTCTTAGCCCACGGCTCTCTATGTCACAATGCGCTAGTCAG |
| Internal bar code: | GTTGTACTGTGCAGTGAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5866 |
| LEAP-Seq percent confirming: | 99.3333 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 150 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGCCATCCGAAAGACAG |
| Suggested primer 2: | AACCATGACACTCAGGCGAG |