| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077251 |
| Chromosome: | chromosome 13 |
| Location: | 1615830 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573450 | CPS | Putative cleavage and polyadenylation specificity factor subunit 1; (1 of 1) K14401 - cleavage and polyadenylation specificity factor subunit 1 (CPSF1, CFT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGGAGGCAGACGCCGCCGCCGCCGCCGCCGCTGAGGGCGCCGCTGC |
| Internal bar code: | TATCTGATAAACGGCAACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATGCATAGTGGCCAGCT |
| Suggested primer 2: | TTCAGCTCTTACACACGCGT |