| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077366 |
| Chromosome: | chromosome 13 |
| Location: | 3328267 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586250 | COA7,COAE2 | Dephospho-CoA kinase, CoA biosynthesis; (1 of 2) 2.7.1.24 - Dephospho-CoA kinase / Dephosphocoenzyme A kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTATGAGGCTGGCCTGCTCCCCGCGTCAGCCTCCGCGCCGCAACGCT |
| Internal bar code: | GGGGGCATGCAGGAAGCGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCCATTCCAATCCCATT |
| Suggested primer 2: | TCATGACAATCTGCCGGCTT |