Insertion junction: CLIP2.077444_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.077444
Chromosome:chromosome 12
Location:5627541
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre12.g531400 NPHP4,NPH4,POC10 (1 of 1) K16478 - nephrocystin-4 (NPHP4); Proteome of centriole protein 10 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTAATTTGCCGGTCAACCTGCTGCGGCGCAACACGGGCTGCAGTCTGG
Internal bar code:CTTTCTCAAAGAATATCTCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:163
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGTGTTGGCGCTTCAA
Suggested primer 2:CAGGTCACGGTTCTTCACGA