| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077474 |
| Chromosome: | chromosome 14 |
| Location: | 1853058 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619825 | (1 of 2) PTHR10848 - MEIOTIC RECOMBINATION PROTEIN SPO11 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCAAGCTTCAATATCATCGCACGAGTCATGCCACACACCCAAGCACGC |
| Internal bar code: | CGGTTATAAGATGACAAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGAACGTGCTACTGCCA |
| Suggested primer 2: | GGGTCGGTGGAAGAAAGGAG |