| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078040 |
| Chromosome: | chromosome 6 |
| Location: | 4708576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279150 | TSD2 | (1 of 2) 6.1.1.12 - Aspartate--tRNA ligase / Aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCTAAATCAATTCGCTAAACTGTCTCTCCTCGCGGCTGCGCGCGCT |
| Internal bar code: | GGGACGTCAGACTTCCGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4280 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTGCTCTCAGTGTTCT |
| Suggested primer 2: | TTCTCAGCAGCCTTGACCTG |