Insertion junction: CLIP2.078656_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.078656
Chromosome:chromosome 12
Location:3976062
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515700 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTAGAGAGTGGATTCTACCGCTTCGGAGCCCCATCATCGGCCATCGCA
Internal bar code:TTTTGACCGGGTGGGATAGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:544
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGCCTCTGATTCCGCAT
Suggested primer 2:CTCTGCTACTCCGGATGCTG