| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.078754 |
| Chromosome: | chromosome 6 |
| Location: | 6292742 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292650 | ROC93 | (1 of 3) K06236 - collagen, type I/II/III/V/XI/XXIV/XXVII, alpha (COL1AS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACACAGCTTGTTTTACGTTTGTAACTTTGTAGGCAGATTGTGACTTTC |
| Internal bar code: | GATCGTGGAAGATGTACTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1579 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCACCGACTACTGCTGCA |
| Suggested primer 2: | CACGAGTTTGCGCAACTGAA |