| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.079176 |
| Chromosome: | chromosome 12 |
| Location: | 546778 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494100 | SPL19 | Pre-mRNA splicing factor; (1 of 1) K12828 - splicing factor 3B subunit 1 (SF3B1, SAP155) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTTCTCGTCCTGTGGGGCCATGTGTGGGGTGTGGGTGGGTGTGGCGT |
| Internal bar code: | GTTTACAGCACCATCCCGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCAAGATCGTGCAGCAG |
| Suggested primer 2: | CAGAGGTGGGGATGAGGAGA |