Insertion junction: CLIP2.079341_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.079341
Chromosome:plastome
Location:172313
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802328 psbJ,2716960,ChreCp063 (1 of 1) K02711 - photosystem II PsbJ protein (psbJ); photosystem II reaction center protein J CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGTATCCCTCTATGGCTTGTTGGTACTGTTGTTGGTACTTTAGCTA
Internal bar code:CTTGTTAAACGAAATAGCGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:71
LEAP-Seq percent confirming:8.33333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCTCACAGTTTGCCAAG
Suggested primer 2:CCCCTTCGGGCAACTAAAGT