| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079431 |
| Chromosome: | chromosome 12 |
| Location: | 3666340 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513500 | XAB2 | (1 of 1) K12867 - pre-mRNA-splicing factor SYF1 (SYF1, XAB2); Transcription-coupled DNA repair protein, possible splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTTTTGTGTTGATGCTTGGTTTGAAGGCTAAGGGTGTGGAGGCATG |
| Internal bar code: | GCTTATATCCTTGGAACCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2367 |
| LEAP-Seq percent confirming: | 28.9474 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCATGCTTTGGCCACACG |
| Suggested primer 2: | ACATGGACGTGGCTGAGAAG |