Insertion junction: LMJ.RY0402.038499_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038499
Chromosome:chromosome_16
Location:2276608
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g659050 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGGCGACCCCTCTGCTGAGGTGGAGGCTG
Internal bar code:GAGTCTCGGCAGGGATGCCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:541
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:215
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTACAGCTACAGCCGCACAT
Suggested primer 2:CAGATGCAGTTCTGGCTGAC