| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038511 |
| Chromosome: | chromosome_1 |
| Location: | 76948 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g000500 | ERR3 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGACGCCGATGGATGCACGCACGCACAGGC |
| Internal bar code: | GAGGTCCTGGATGCTCCCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.7084 |
| LEAP-Seq n confirming: | 5129 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACTAATTCACCAACCCT |
| Suggested primer 2: | TTAGCCATACTGAGCCGCTT |