| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038539 |
| Chromosome: | chromosome_1 |
| Location: | 4814611 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g033350 | ALD2 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTACCGCTCCCCAAGCACACGCACGGGTC |
| Internal bar code: | ACGAGGCTCCGCTCGTCGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1262 |
| LEAP-Seq percent confirming: | 99.6041 |
| LEAP-Seq n confirming: | 16353 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGGGAAGCATAGGAC |
| Suggested primer 2: | CCAGCACCCAGTGGTCTTAT |