Insertion junction: LMJ.RY0402.038539_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038539
Chromosome:chromosome_1
Location:4814612
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre01.g033350 ALD2 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TGGCATTGAAGGAAGAAGTGTTCTTACACG
Internal bar code:AGCCGATCTATGGATAAAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1008
LEAP-Seq percent confirming:99.4863
LEAP-Seq n confirming:2324
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAGGGGAAGCATAGGAC
Suggested primer 2:CCAGCACCCAGTGGTCTTAT