Insertion junction: LMJ.RY0402.038594_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.038594
Chromosome:chromosome_13
Location:2254714
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g578750 TBA1 PsbA translation factor antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTATGTCCTCGGACCGCCTGGAGACAAGAC
Internal bar code:GGTGTATCTGGAATGTTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1084
LEAP-Seq percent confirming:99.4092
LEAP-Seq n confirming:8245
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGAAGCAACGACGGATAC
Suggested primer 2:CAGAAGAAGAGGAACCCGTG