Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.038594 |
Chromosome: | chromosome_13 |
Location: | 2254714 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g578750 | TBA1 | PsbA translation factor | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTATGTCCTCGGACCGCCTGGAGACAAGAC |
Internal bar code: | GGTGTATCTGGAATGTTTTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1084 |
LEAP-Seq percent confirming: | 99.4092 |
LEAP-Seq n confirming: | 8245 |
LEAP-Seq n nonconfirming: | 49 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGAAGCAACGACGGATAC |
Suggested primer 2: | CAGAAGAAGAGGAACCCGTG |