| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038806 |
| Chromosome: | chromosome_16 |
| Location: | 6762458 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g673617 | PRF1 | Peptide chain release factor | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCACACCGCATGCGAGGACTCAACCCTA |
| Internal bar code: | GTACGCTGGTCGGACTGGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.6805 |
| LEAP-Seq n confirming: | 3120 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTGTGGGGACTCACAGT |
| Suggested primer 2: | GAACATTTGGTTGACGCCTT |