Gene: Cre16.g673617 (Genome version: v5.5)

Overview

Common name:PRF1
Defline:Peptide chain release factor
Description:Mitochondrial peptide chain release factor 1, similar to eubacterial class 1 release factors (prfA, RF1); (reliability class 3 by TargetP). Previously annotated as PRFA1
Synonyms:g16713.t1,Cre01.g065550.t1.1,Cre01.g065550.t1.2,PRF1
Chromosome:chromosome_16

Protein Localization

A plasmid for expressing Cre16.g673617 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre16.g673617-Venus-3xFLAG

Imaged strain: CSI_LW09A7

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/pyrenoid depleted

cytosol/cytosol (strong signal)

nucleus/nucleoplasm

nucleus/nucleolus

(with similar signal in chloroplast, cytosol, and nucleus)

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Orientation Insertion Junction Feature Confidence (%)
LMJ.RY0402.038806_1 LMJ.RY0402.038806 antisense 3'UTR 73
LMJ.RY0402.043647_1 LMJ.RY0402.043647 sense 3'UTR 58
LMJ.RY0402.157031_1 LMJ.RY0402.157031 sense 3'UTR 95
LMJ.RY0402.157031_2 LMJ.RY0402.157031 sense 3'UTR 95

Gene Annotations

PFAM:PF03462, PF00472
PANTHER: PTHR11075:SF13 PTHR11075
KEGG_ec:
KEGG_orthology:K02835
KOG:KOG2726
Gene ontology terms::GO:0005737, GO:0006415, GO:0016149, GO:0003747
Best arabidopsis TAIR name:AT3G62910.1
Best arabidopsis TAIR symbol:APG3
Best arabidopsis TAIR defline:Peptide chain release factor 1
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.