| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157031 |
| Chromosome: | chromosome 16 |
| Location: | 6761960 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673617 | PRF1,PRFA1 | Putative chloroplast peptide chain release factor 1; (1 of 2) PTHR11075:SF9 - PEPTIDE CHAIN RELEASE FACTOR 1, MITOCHONDRIAL-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGTCCAGCGGAGCAACCGATATTTAAG |
| Internal bar code: | TATCTGGTTACCACTATCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 95.0368 |
| LEAP-Seq n confirming: | 517 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGGAGCGTGTGCTCTTG |
| Suggested primer 2: | AAACCCAACAGGAAGTGTGC |