Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.039473 |
Chromosome: | chromosome_4 |
Location: | 2957527 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g224800 | VAMP74,VAM4 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCATCGGCACACCGTAGACAGTCCATCACT |
Internal bar code: | TGGGCCGCCGTCTGCGCACGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1037 |
LEAP-Seq percent confirming: | 99.4594 |
LEAP-Seq n confirming: | 10303 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAAGGTTTTGTCCGTCAGG |
Suggested primer 2: | GTGTTTGGTTGCATGTTTGC |