| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039482 |
| Chromosome: | chromosome 7 |
| Location: | 3489803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335750 | IFT88 | (1 of 1) K16474 - intraflagellar transport protein 88 (IFT88); Intraflagellar Transport Protein 88 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATAGTGTCATGGATATGTGGCCTCATG |
| Internal bar code: | GTATGATTCATTTTTAGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1124 |
| LEAP-Seq percent confirming: | 99.0192 |
| LEAP-Seq n confirming: | 2726 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGCATGCAAGAGGATA |
| Suggested primer 2: | TCCGAGAAGGTAGGAGGGTT |