| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039482 |
| Chromosome: | chromosome 7 |
| Location: | 3489808 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335750 | IFT88 | (1 of 1) K16474 - intraflagellar transport protein 88 (IFT88); Intraflagellar Transport Protein 88 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGCTGAAGTGTGCTGCAGGTGCGACCC |
| Internal bar code: | TCGCGGTAGGTAGTCTTGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 72 |
| LEAP-Seq percent confirming: | 99.9021 |
| LEAP-Seq n confirming: | 1020 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGCATGCAAGAGGATA |
| Suggested primer 2: | TCCGAGAAGGTAGGAGGGTT |