| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039912 |
| Chromosome: | chromosome 12 |
| Location: | 1173187 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489900 | CYG54 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGGTAAAACAGCGGCTGTAGCGCTCAC |
| Internal bar code: | CTGCGTGGCTGTAGTGGCGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1247 |
| LEAP-Seq percent confirming: | 99.5633 |
| LEAP-Seq n confirming: | 2964 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGGCCTTGATTCTATGT |
| Suggested primer 2: | TGGTAAGTGACGCCCATACA |