Insertion junction: LMJ.RY0402.041083_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.041083
Chromosome:chromosome_1
Location:7071175
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre01.g051050 FAP225 Flagellar Associated Protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TGTCCGCAGTACTAAGCACATCGTACGCAA
Internal bar code:CCCGTACAGTCCCCGCACCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:796
LEAP-Seq percent confirming:99.3194
LEAP-Seq n confirming:5691
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGATTCAGAGGCACGAA
Suggested primer 2:CACGCGACCCACATACATAG