| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041083 |
| Chromosome: | chromosome 1 |
| Location: | 7071182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051050 | FAP225 | (1 of 18) PTHR10891 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN; Flagellar Associated Protein 225 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCAAAAGCGGATGCCACATGGGCACA |
| Internal bar code: | CCCCGACCACAATCTAGGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.5355 |
| LEAP-Seq n confirming: | 5357 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGATTCAGAGGCACGAA |
| Suggested primer 2: | CACGCGACCCACATACATAG |