Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.041190 |
Chromosome: | chromosome 16 |
Location: | 1240090 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g651050 | CYC6,PETJ | (1 of 2) K08906 - cytochrome c6 (petJ); Cytochrome c6 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGGGTCCGCTTTGTCACAGGCTTGCTT |
Internal bar code: | GTGTCGTGATCCCTCGGGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 857 |
LEAP-Seq percent confirming: | 98.2898 |
LEAP-Seq n confirming: | 8046 |
LEAP-Seq n nonconfirming: | 140 |
LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGTTGTTGGTGCTGATTG |
Suggested primer 2: | GCTTCAAGGTGGAGAGCATC |