| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041595 |
| Chromosome: | chromosome 13 |
| Location: | 2469197 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGATCAGGCCCAGGAAGGAGTTGCGGA |
| Internal bar code: | CCTGCTTACGGTTGTGCCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 98.312 |
| LEAP-Seq n confirming: | 4077 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTCTTTCTCAGCCGAA |
| Suggested primer 2: | GTCCACCACGCTCGTTATTT |