| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041695 |
| Chromosome: | chromosome 13 |
| Location: | 2468520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCTTCGCCCTTGTTCGCTGTGTCACTC |
| Internal bar code: | TGTTCCGGCGCGACGTCTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1244 |
| LEAP-Seq percent confirming: | 99.2848 |
| LEAP-Seq n confirming: | 7774 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACATGCAGCAGAGGGAG |
| Suggested primer 2: | GCCTCTATCCCTTCCCTCAC |