| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041731 |
| Chromosome: | chromosome_3 |
| Location: | 3049262 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g164250 | FAP262 | Flagellar Associated Protein, protein kinase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGGCTGCGCCTCTGCCCCTGCCTCCGCCG |
| Internal bar code: | CCTTCGTTTTGGGACGCGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 99.6927 |
| LEAP-Seq n confirming: | 11032 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAATGGTGTACCGCGAC |
| Suggested primer 2: | CATATGCTTGCGTGTGCTCT |