Insertion junction: LMJ.RY0402.041841_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.041841
Chromosome:chromosome 9
Location:5833533
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g402219 LCI3 Low-CO2-inducible protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCTCACATCACGCGGTTTCGGCTCGCA
Internal bar code:TGCTTAGTCCCCACTGTCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:877
LEAP-Seq percent confirming:99.5226
LEAP-Seq n confirming:3544
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGAGTGGCTGCTAAACCT
Suggested primer 2:ATGATCCACCAGTGTGCGTA