| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042043 |
| Chromosome: | chromosome 2 |
| Location: | 747026 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078507 | (1 of 1) IPR006311//IPR025585 - Twin-arginine translocation pathway, signal sequence // Photosystem II Pbs27 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATACTTGTCACTTCAACTACAAAGCGTT |
| Internal bar code: | CGGAAGAACCGAATGCGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 99.2958 |
| LEAP-Seq n confirming: | 1410 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCATACCATCAAGCAG |
| Suggested primer 2: | CTTTCAGGTGCTTCGCCTAC |