| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042450 |
| Chromosome: | chromosome_4 |
| Location: | 675336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217951 | LCI33 | Low-CO2-inducible protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCCATCCCCATGTGGCAAGCAATGAGAA |
| Internal bar code: | TTAGTGGAAAGGAAAATTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.1137 |
| LEAP-Seq n confirming: | 671 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAACAAGCTCATCGAC |
| Suggested primer 2: | GAGTCAAAGCTAACCGCGTC |