Insertion junction: LMJ.RY0402.042518_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.042518
Chromosome:chromosome 12
Location:5544888
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g531400 NPHP4,NPH4,POC10 (1 of 1) K16478 - nephrocystin-4 (NPHP4); Proteome of centriole protein 10 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCTCGCGCAAGAGCGCACGCGTACAGCA
Internal bar code:TGGGCCTTCAGACAACCCCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:537
LEAP-Seq percent confirming:99.1652
LEAP-Seq n confirming:7246
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTAATGCGCAGCTCAAAC
Suggested primer 2:TCAATCATCTCCAAGGGCTC