| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042606 |
| Chromosome: | chromosome 5 |
| Location: | 449948 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232550 | GPM4,PGM4,CA1P1 | (1 of 1) 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase; 2-Carboxy-D-arabinitol-1-phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTCGGGTTGGCGGGGTCCAGGAGAAGG |
| Internal bar code: | CCCAAAGCGTTGAGCCCGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 99.5766 |
| LEAP-Seq n confirming: | 1176 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACACGGATGACTCGACA |
| Suggested primer 2: | GGCCGAGACCAAACGTATTA |