| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042720 |
| Chromosome: | chromosome 1 |
| Location: | 74264 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000500 | AXL5,ERR3 | Arabinose chain extension enzyme like protein 5; (1 of 6) PTHR10994//PTHR10994:SF61 - RETICULON // RETICULON-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACATGTCAGGATGGTACACCTGTCGGC |
| Internal bar code: | TGGTTTTAAAGTCTTTCATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 99.3233 |
| LEAP-Seq n confirming: | 1321 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGATGCCTTCTTTGGGA |
| Suggested primer 2: | GAACAGGGTCACTCGTCCAT |