| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042744 |
| Chromosome: | chromosome 1 |
| Location: | 7057484 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050950 | CHLP1,CHLP,LYL1 | Geranylgeranyl reductase CHLP; (1 of 1) 1.3.1.83 - Geranylgeranyl diphosphate reductase / Geranylgeranyl reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGGCTCGAACAGAGCCAGCAGGGCGC |
| Internal bar code: | GCCTCGTGTGTTTGTTTGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 98.9637 |
| LEAP-Seq n confirming: | 191 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGCCTGATAACGCAAG |
| Suggested primer 2: | TTACAGCAGGCTAGGCGTTT |