Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.043117 |
Chromosome: | chromosome_4 |
Location: | 676723 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g217951 | LCI33 | Low-CO2-inducible protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTACAGACTCTGTGCCTCAACATGCAGCCA |
Internal bar code: | TCCTGGGTCCTACTGGACATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1155 |
LEAP-Seq percent confirming: | 99.1693 |
LEAP-Seq n confirming: | 9073 |
LEAP-Seq n nonconfirming: | 76 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACGCGGTTAGCTTTGACTC |
Suggested primer 2: | GCTGACGGGATAAAGATGGA |