| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044057 |
| Chromosome: | chromosome 10 |
| Location: | 1164169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425900 | LHCA5 | (1 of 2) K08910 - light-harvesting complex I chlorophyll a/b binding protein 4 (LHCA4); Light-harvesting protein 5 of photosystem I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTGTATACCCAAGCTTTTTGAGGCTT |
| Internal bar code: | GGGTGGTGTGTGGGTTTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 98.9712 |
| LEAP-Seq n confirming: | 481 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAAGAACTTCGGCTCGG |
| Suggested primer 2: | CGGTCAACAATCGAGGTTTT |