| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.044913 |
| Chromosome: | chromosome 9 |
| Location: | 5572747 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400812 | (1 of 2) PF12325 - TATA element modulatory factor 1 TATA binding (TMF_TATA_bd) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCTGGGCCTGCTGGCGCTCTTGCTCCG |
| Internal bar code: | ATTGGTGGGGCAGAGGCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 96.291 |
| LEAP-Seq n confirming: | 675 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCTGGTTTGTGTGATT |
| Suggested primer 2: | CGGGGAACACTTGTCTCAAT |