| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045316 |
| Chromosome: | chromosome 6 |
| Location: | 7323703 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298850 | LTH6 | LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGGGCGGCTTCTCCTCCGTGCCGCTG |
| Internal bar code: | TCTGGGGGAATTTCGGAGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 99.1037 |
| LEAP-Seq n confirming: | 774 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAATGTGGTGGACATCAG |
| Suggested primer 2: | CACATCAAGACAAGAGGCCA |