| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045651 |
| Chromosome: | chromosome_1 |
| Location: | 870681 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g004550 | FAP190 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTAGGCAACCCTGCCTGGGAATGACATGCG |
| Internal bar code: | TCGTGTTGGTAGTTGATTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 99.2945 |
| LEAP-Seq n confirming: | 563 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGATGAGCCTTGCTAA |
| Suggested primer 2: | AGCGGAAGAACCAGTGCTAA |