Insertion junction: LMJ.RY0402.045932_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.045932
Chromosome:chromosome 4
Location:3780933
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCAGTGGCGGCGCCAACAGCAACAGTAG
Internal bar code:CAGCAACCGGCCCTCGCGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:684
LEAP-Seq percent confirming:90.4478
LEAP-Seq n confirming:10160
LEAP-Seq n nonconfirming:1073
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATCATGACGTCCTTACC
Suggested primer 2:TCGAAGTGCTCCTCAAAGGT