| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045961 |
| Chromosome: | chromosome 16 |
| Location: | 2084015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657550 | (1 of 1) PF08553 - VID27 cytoplasmic protein (VID27) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAACCCTCCCAGTCCACCCAGCCCTGA |
| Internal bar code: | GTGGGATCGCTGGGTGGAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 98.6792 |
| LEAP-Seq n confirming: | 1046 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCAGCCATACTCTCTCG |
| Suggested primer 2: | CTCTGCTGAGGCCAGTTACC |