| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046013 |
| Chromosome: | chromosome 16 |
| Location: | 5197717 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683050 | RNJ1 | (1 of 1) 3.5.2.6 - Beta-lactamase / Penicillinase; chloroplast RNase J | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGTAACTAGTACCTGTGAGCTACGCG |
| Internal bar code: | GTGCTAGCGTCTATTATGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.9206 |
| LEAP-Seq n confirming: | 1258 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCAGTGACTTGTCCGTT |
| Suggested primer 2: | CCGATTACGGCTAAGGTGTC |