| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046218 |
| Chromosome: | chromosome 10 |
| Location: | 2218022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433950 | (1 of 2) PTHR33222:SF3 - PROTEIN CURVATURE THYLAKOID 1C, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCAAAGGTTGCGGGCCTATGCTCGCTG |
| Internal bar code: | GTGCCGCGGCTGGTGGGCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 98.5298 |
| LEAP-Seq n confirming: | 8109 |
| LEAP-Seq n nonconfirming: | 121 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTTGCGGTTTCAGAGGA |
| Suggested primer 2: | CGTCTTTCTCGGACTTTTGC |